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Issue 76, 2019
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Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

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Abstract

Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enabling simultaneous quantification of the lesions on individual DNA molecules. We follow the repair of both damage types in skin cells exposed to artificial sunlight.

Graphical abstract: Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

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Publication details

The article was received on 07 Jul 2019, accepted on 26 Aug 2019 and first published on 02 Sep 2019


Article type: Communication
DOI: 10.1039/C9CC05198H
Chem. Commun., 2019,55, 11414-11417

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    Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

    D. Torchinsky, Y. Michaeli, N. R. Gassman and Y. Ebenstein, Chem. Commun., 2019, 55, 11414
    DOI: 10.1039/C9CC05198H

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