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Clickable PEG hydrogel microspheres as building blocks for 3D bioprinting

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Abstract

Three-dimensional (3D) bioprinting is important in the development of complex tissue structures for tissue engineering and regenerative medicine. However, the materials used for bioprinting, referred to as bioinks, must have a balance between a high viscosity for rapid solidification after extrusion and low shear force for cytocompatibility, which is difficult to achieve. Here, a novel bioink consisting of poly(ethylene glycol) (PEG) microgels prepared via off-stoichiometry thiol–ene click chemistry is introduced. Importantly, the microgel bioink is easily extruded, exhibits excellent stability after printing due to interparticle adhesion forces, and can be photochemically annealed with a second thiol–ene click reaction to confer long-term stability to printed constructs. The modularity of the bioink is also an advantage, as the PEG microgels have highly tunable physicochemical properties. The low force required for extrusion and cytocompatibility of the thiol–ene annealing reaction also permit cell incorporation during printing with high viability, and cells are able to spread and proliferate in the interstitial spaces between the microgels after the constructs have been annealed. Overall, these results indicate that our microgel bioink is a promising and versatile platform that could be leveraged for bioprinting and regenerative manufacturing.

Graphical abstract: Clickable PEG hydrogel microspheres as building blocks for 3D bioprinting

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Publication details

The article was received on 12 Oct 2018, accepted on 09 Jan 2019 and first published on 09 Jan 2019


Article type: Paper
DOI: 10.1039/C8BM01286E
Citation: Biomater. Sci., 2019, Advance Article
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    Clickable PEG hydrogel microspheres as building blocks for 3D bioprinting

    S. Xin, D. Chimene, J. E. Garza, A. K. Gaharwar and D. L. Alge, Biomater. Sci., 2019, Advance Article , DOI: 10.1039/C8BM01286E

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