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Issue 7, 2019
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Luminescence thermometry for in situ temperature measurements in microfluidic devices

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Abstract

Temperature control for lab-on-a-chip devices has resulted in the broad applicability of microfluidics to, e.g., polymerase chain reaction (PCR), temperature gradient focusing for electrophoresis, and colloidal particle synthesis. However, currently temperature sensors on microfluidic chips either probe temperatures outside the channel (resistance temperature detector, RTD) or are limited in both the temperature range and sensitivity in the case of organic dyes. In this work, we introduce ratiometric bandshape luminescence thermometry in which thermally coupled levels of Er3+ in NaYF4 nanoparticles are used as a promising method for in situ temperature mapping in microfluidic systems. The results, obtained with three types of microfluidic devices, demonstrate that temperature can be monitored inside a microfluidic channel accurately (0.34 °C) up to at least 120 °C with a spot size of ca. 1 mm using simple fiber optics. Higher spatial resolution can be realized by combining luminescence thermometry with confocal microscopy, resulting in a spot size of ca. 9 μm. Further improvement is anticipated to enhance the spatial resolution and allow for 3D temperature profiling.

Graphical abstract: Luminescence thermometry for in situ temperature measurements in microfluidic devices

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Publication details

The article was received on 27 Nov 2018, accepted on 20 Feb 2019 and first published on 25 Feb 2019


Article type: Paper
DOI: 10.1039/C8LC01292J
Lab Chip, 2019,19, 1236-1246

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    Luminescence thermometry for in situ temperature measurements in microfluidic devices

    R. G. Geitenbeek, J. C. Vollenbroek, H. M. H. Weijgertze, C. B. M. Tregouet, A. Nieuwelink, C. L. Kennedy, B. M. Weckhuysen, D. Lohse, A. van Blaaderen, A. van den Berg, M. Odijk and A. Meijerink, Lab Chip, 2019, 19, 1236
    DOI: 10.1039/C8LC01292J

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