Jump to main content
Jump to site search

Issue 76, 2019
Previous Article Next Article

Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

Author affiliations

Abstract

Herein we present an assay allowing concurrent detection of oxidative DNA damage and photoproducts. We apply DNA repair enzymes specific for each lesion type to incorporate spectrally distinct fluorescent nucleotides, enabling simultaneous quantification of the lesions on individual DNA molecules. We follow the repair of both damage types in skin cells exposed to artificial sunlight.

Graphical abstract: Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

Back to tab navigation

Supplementary files

Article information


Submitted
07 Jul 2019
Accepted
26 Aug 2019
First published
02 Sep 2019

Chem. Commun., 2019,55, 11414-11417
Article type
Communication

Simultaneous detection of multiple DNA damage types by multi-colour fluorescent labelling

D. Torchinsky, Y. Michaeli, N. R. Gassman and Y. Ebenstein, Chem. Commun., 2019, 55, 11414
DOI: 10.1039/C9CC05198H

Social activity

Search articles by author

Spotlight

Advertisements