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Issue 24, 2019
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A coumarin–hemicyanine hybrid as a ratiometric fluorescent sensor of microenvironment proticity

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Abstract

The coumarin–hemicyanine hybrid is an internal charge transfer (ICT) dye that features a donor-π-acceptor motif and displays two well-resolved absorption bands at ∼350 nm for the donor coumarin and ∼550 nm for the fully-conjugated dye. Herein, we demonstrate that preferential excitation of the dye at 350 nm, ascribed to a twisted conformation, gives rise to two well-resolved emission bands at ∼450 nm for the coumarin and ∼650 nm for the planar dye that are highly quenched in water. Unexpectedly, the coumarin blue emission was found to ‘turn-on’ to aprotic and viscous solvents and respond to binding bovine serum albumin (BSA) in aqueous media. In contrast, the red ICT emission was sensitive to protic solvent polarity and responded to binding the G-quadruplex (GQ) DNA structure produced by the heme binding aptamer (PS2.M). Our studies also demonstrate the influence of the alkyl group attached to the dye quaternary nitrogen atom in determining the microenvironment sensitivity and ability to differentiate biopolymer types.

Graphical abstract: A coumarin–hemicyanine hybrid as a ratiometric fluorescent sensor of microenvironment proticity

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Publication details

The article was received on 21 Dec 2018, accepted on 27 Feb 2019 and first published on 28 Feb 2019


Article type: Communication
DOI: 10.1039/C8CC10132A
Chem. Commun., 2019,55, 3540-3543

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    A coumarin–hemicyanine hybrid as a ratiometric fluorescent sensor of microenvironment proticity

    P. S. Deore, D. S. Coman and R. A. Manderville, Chem. Commun., 2019, 55, 3540
    DOI: 10.1039/C8CC10132A

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