Chimeric crRNAs with 19 DNA residues in the guide region show the retained DNA cleavage activity of Cas9 with potential to improve the specificity

Hyo Young Kim; Seong Jae Kang; Yongmoon Jeon; Jinsu An; Jihyun Park; Hee Jae Lee; Jeong-Eun Jang; JongSeong Ahn; Duhee Bang; Hak Suk Chung; Cherlhyun Jeong; Dae-Ro Ahn

doi:10.1039/C8CC08468H

Chimeric crRNAs with 19 DNA residues in the guide region show the retained DNA cleavage activity of Cas9 with potential to improve the specificity†

Hyo Young Kim,‡^a Seong Jae Kang,‡^a Yongmoon Jeon,^a Jinsu An,^ab Jihyun Park,^a Hee Jae Lee,^a Jeong-Eun Jang,^a JongSeong Ahn,^c Duhee Bang,^c Hak Suk Chung,^ab Cherlhyun Jeong^a and Dae-Ro Ahn

*^ab

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* Corresponding authors

^a Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea
E-mail: drahn@kist.re.kr

^b Division of Biomedical Science and Technology, Korea University of Science and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Korea

^c Department of Chemistry, College of Science, Yonsei University, Republic of Korea

Abstract

We demonstrated that 19 out of 20 RNA residues in the guide region of crRNA can be replaced with DNA residues with high GC-contents. The cellular activity of the chimeric crRNAs to disrupt the target gene was comparable to that of the native crRNA.

Chemical Communications

Chimeric crRNAs with 19 DNA residues in the guide region show the retained DNA cleavage activity of Cas9 with potential to improve the specificity†

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Chimeric crRNAs with 19 DNA residues in the guide region show the retained DNA cleavage activity of Cas9 with potential to improve the specificity

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