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Issue 43, 2018
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Ratiometric fluorescent probes for capturing endogenous hypochlorous acid in the lungs of mice

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Abstract

Hypochlorous acid (HClO) is a promising diagnostic marker for inflammation and relevant diseases. Although many probes were previously developed for HClO imaging, the development of organ targeting probes is still lacking. Herein, we designed and synthesized a series of cyanine derivatives as ratiometric fluorescent probes to detect endogenous HClO in the lungs with inflammation. By installing diverse lipid chains and amino groups on cyanine, we identified that ClO1, with one n-octadecane chain and two 2-[[2-(dimethylamino)ethyl]methylamino]-ethyl groups, is a superior probe to target the lungs over other major organs in mice. ClO1 was able to sense both exogenous and endogenous HClO in A549 (human lung epithelial) cells through fluorescence ratiometric imaging. In a lipopolysaccharide (LPS)-induced lung inflammation mouse model, ClO1 effectively captured endogenous HClO in the lungs after intravenous administration. Overall, these cyanine-derived probes merit further development as organ targeting HClO sensors.

Graphical abstract: Ratiometric fluorescent probes for capturing endogenous hypochlorous acid in the lungs of mice

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Publication details

The article was received on 20 Jul 2018, accepted on 04 Sep 2018 and first published on 05 Sep 2018


Article type: Edge Article
DOI: 10.1039/C8SC03226B
Citation: Chem. Sci., 2018,9, 8207-8212
  • Open access: Creative Commons BY-NC license
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    Ratiometric fluorescent probes for capturing endogenous hypochlorous acid in the lungs of mice

    X. Zhang, W. Zhao, B. Li, W. Li, C. Zhang, X. Hou, J. Jiang and Y. Dong, Chem. Sci., 2018, 9, 8207
    DOI: 10.1039/C8SC03226B

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