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Issue 3, 2019
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pH-Dependent fluorescence from firefly oxyluciferin in agarose thin films

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Abstract

The yellow-green light emitted by fireflies is one of the most prominent examples of bioluminescence. Firefly oxyluciferin, the emitting molecule, is labile in alkaline solutions, and its structure is strongly affected by solvent polarity and pH. Previous studies have suggested that variations in the active site conditions are likely contributors to the color of bioluminescent emission. Herein, we incorporate firefly oxyluciferin into an agarose matrix to emulate the enzyme active site. Self-supporting, lightweight thin films were fabricated by solution casting and spectroscopically characterized. The previously described acidochromism of oxyluciferin is conserved in the thin films. The bathochromic shift observed in alkaline conditions results from the formation of the oxyluciferin dianion. This study demonstrates an alternative approach to investigating environmental effects on bioluminescent molecules.

Graphical abstract: pH-Dependent fluorescence from firefly oxyluciferin in agarose thin films

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Supplementary files

Article information


Submitted
28 Oct 2018
Accepted
26 Nov 2018
First published
27 Nov 2018

New J. Chem., 2019,43, 1122-1126
Article type
Letter

pH-Dependent fluorescence from firefly oxyluciferin in agarose thin films

N. M. Lui, S. Schramm and P. Naumov, New J. Chem., 2019, 43, 1122
DOI: 10.1039/C8NJ05469J

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