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Trypsin-calcium carbonate hybrid nanospheres based enzyme reactor with good stability and reusability

Abstract

Controlled fabrication of CaCO3 nanoparticles with exquisite polymorph and morphology has received intensive attention due to its broad industrial and biomedical applications. Herein we have created trypsin-CaCO3 hybrid nanoparticles with well-controlled morphology and crystal phase via very mild biomineralization process. The incubation of the mixture of sodium carbonate and calcium chloride at room temperature using trypsin as the biotemplate leads to predominantly calcite mesocrystals with porous sphere-like nanostructures. The trypsin-CaCO3 hybrid nanospheres behave as superior nano-biocatalysts, which display high stability against thermal and chemical denaturation as well as excellent reusability. We have demonstrated that trypsin not only provides a novel robust biotemplate to mediate the growth of CaCO3 crystals, but also enhance its biological properties as an excellent enzyme. It indicates that biomineralization may provide a facile and environmentally amiable approach to immobile enzymes on calcium carbonate nanoparticles. Our studies would greatly enhance our understanding of the mechanism of protein-mediated biomineralization of CaCO3, and extend the applications of CaCO3-based composite nanostructured biomaterials.

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Publication details

The article was received on 22 Aug 2018, accepted on 11 Oct 2018 and first published on 11 Oct 2018


Article type: Paper
DOI: 10.1039/C8NJ04282A
Citation: New J. Chem., 2018, Accepted Manuscript
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    Trypsin-calcium carbonate hybrid nanospheres based enzyme reactor with good stability and reusability

    J. C. Munyemana, H. He, C. Fu, W. Wei, J. Tian and J. Xiao, New J. Chem., 2018, Accepted Manuscript , DOI: 10.1039/C8NJ04282A

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