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A sensitive detection method of carcinoembryonic antigen based on dsDNA-templated copper nanoparticles

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Abstract

As a tumor marker, carcinoembryonic antigen (CEA) is an effective indicator for the evaluation of the response to treatments of cancers. Therefore, it is necessary to find some sensitive and effective methods for CEA detection. Herein, we describe a facile method of CEA determination based on dsDNA-templated copper nanoparticles (CuNPs) coupled with an aptamer. In this fluorescence bioassay, the dsDNA residue contains a CEA-specific aptamer that seizes the formation of fluorescent CuNPs in the presence of CEA, resulting in weak fluorescence emission. In the absence of CEA, the dsDNA-templated CuNPs can generate strong fluorescence with emission peaks at 565 nm. This assay enables a sensitive and precise determination of CEA with a detection limit of 0.0065 ng mL−1 (S/N = 3) and functions successfully in biological samples.

Graphical abstract: A sensitive detection method of carcinoembryonic antigen based on dsDNA-templated copper nanoparticles

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Publication details

The article was received on 04 Jun 2018, accepted on 09 Jul 2018 and first published on 10 Jul 2018


Article type: Paper
DOI: 10.1039/C8NJ02774A
Citation: New J. Chem., 2018, Advance Article
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    A sensitive detection method of carcinoembryonic antigen based on dsDNA-templated copper nanoparticles

    M. Chen, F. Yeasmin Khusbu, C. Ma, K. Wu, H. Zhao, H. Chen and K. Wang, New J. Chem., 2018, Advance Article , DOI: 10.1039/C8NJ02774A

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