Issue 5, 2018

Sensitive detection of Japanese encephalitis virus by surface molecularly imprinted technique based on fluorescent method

Abstract

This study demonstrated fluorescent detection of Japanese encephalitis virus (JEV) by surface molecularly imprinted technique. After dansyl chloride (DNS-Cl) was immobilized on silica microspheres (SiMP) with JEV as the template, (3-aminopropyl)triethoxysilane (APTES) as the monomer and tetraethyl orthosilicate (TEOS) as the cross-linker was mixed with SiMP, and the polymerization was performed in mild condition. After polymerization, templates were removed to obtain JEV-imprinted polymers (JEV-MIPs). The obtained JEV-MIPs can selectively recognize JEV in the presence of Hepatitis A virus (HAV), Simian virus 40 (SV40) and Rabies virus (RV), and also sensitively detect JEV with a picomolar detection limit.

Graphical abstract: Sensitive detection of Japanese encephalitis virus by surface molecularly imprinted technique based on fluorescent method

Article information

Article type
Paper
Submitted
05 Dec 2017
Accepted
19 Jan 2018
First published
19 Jan 2018

New J. Chem., 2018,42, 3503-3508

Sensitive detection of Japanese encephalitis virus by surface molecularly imprinted technique based on fluorescent method

W. Feng, C. Liang, H. Gong and C. Cai, New J. Chem., 2018, 42, 3503 DOI: 10.1039/C7NJ04791F

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