Jump to main content
Jump to site search


Advances in design of cell-permeable fluorescent probe for applications in live cell imaging

Abstract

Small fluorescent molecule has been an important tool for fluorescence-based imaging, thanks to its technical simplicity, sensitivity and structural flexibility. However, probing specific intracellular system with small fluorescent molecule is not an easy task due to the intricate nature of the cells. Ideal imaging probes should be highly permeable without background noise to warrant undisrupted observation. These probes will unquestionably be more advantageous to interrogate intracellular architectures and dynamics without compromising cellular integrity, thus be suitably applied for applications in native cellular environment. This review will highlight the advances in design strategy for cell-permeable fluorescent probes through diversity-oriented fluorescence library approach and rational design via computational-aided predictive model. Here we discuss a series of cell-permeable probes applications in imaging specific cell types, intracellular biomolecules as well as cellular environment.

Back to tab navigation

Publication details

The article was received on 10 Oct 2018, accepted on 07 Nov 2018 and first published on 07 Nov 2018


Article type: Feature Article
DOI: 10.1039/C8CC08107G
Citation: Chem. Commun., 2018, Accepted Manuscript
  •   Request permissions

    Advances in design of cell-permeable fluorescent probe for applications in live cell imaging

    S. H. Alamudi and Y. Chang, Chem. Commun., 2018, Accepted Manuscript , DOI: 10.1039/C8CC08107G

Search articles by author

Spotlight

Advertisements