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Issue 2, 2017
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IGF-2 coated porous collagen microwells for the culture of pancreatic islets

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Abstract

Islet transplantation, the only curative therapy for type I diabetes, requires isolation of the graft in highly specialized facilities for its later dispatch to remote transplantation centres. During transport and culture, many valuable cells are lost due to several factors such as mechanical stress, islet aggregation and dissociation. Here, we evaluate a porous microwell array sheet made of natural collagen type I extracellular matrix (ECM) protein as a novel islet culture substrate. This culture platform can be coated with IGF-2, a growth factor favorable for islet survival, and allows segregation of the islets within the porous microwell sheet, preventing aggregation. This design shows promising results for improving human pancreatic islets viability and function during culture and could form a novel paradigm for the transport of islets between isolation and transplantation centres.

Graphical abstract: IGF-2 coated porous collagen microwells for the culture of pancreatic islets

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Publication details

The article was received on 21 Oct 2016, accepted on 12 Dec 2016 and first published on 20 Dec 2016


Article type: Communication
DOI: 10.1039/C6TB02748B
Citation: J. Mater. Chem. B, 2017,5, 220-225
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    IGF-2 coated porous collagen microwells for the culture of pancreatic islets

    A. Forget, M. Waibel, D. M. Rojas-Canales, S. Chen, N. Kawazoe, F. J. Harding, T. Loudovaris, P. T. H. Coates, A. Blencowe, G. Chen and N. H. Voelcker, J. Mater. Chem. B, 2017, 5, 220
    DOI: 10.1039/C6TB02748B

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