Jump to main content
Jump to site search
SCHEDULED MAINTENANCE Close the message box

Maintenance work is planned for Monday 16 August 2021 from 07:00 to 23:59 (BST).

Website performance may be temporarily affected and you may not be able to access some PDFs or images. If this does happen, refreshing your web browser should resolve the issue. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 47, 2017

Optical control of mitochondrial reductive reactions in living cells using an electron donor–acceptor linked molecule

Author affiliations

Abstract

It has been known for decades that intracellular redox reactions control various vital functions in living systems, which include the synthesis of biomolecules, the modulation of protein functions, and cell signaling. Although there have been several reports on the control of such functions using DNA and RNA, the non-invasive optical control of biological functions is an important ongoing challenge. In this study, a hybrid of an electron donor–acceptor linked molecule based on a ferrocene(Fc)–porphyrin(ZnP)–fullerene(C60) analogue and an elaborately designed nano-carrier, referred to herein as a MITO-Porter, resulted in a successful photoinduced intermolecular electron transfer reaction via the long-lived intramolecular charge separation, leading to site-specific reductive reactions in the mitochondria of living HeLa cells. A Fc-ZnP-C60 linked molecule, 1-Oct, was designed and prepared for taking advantage of the unique photophysical properties with excellent efficiency (i.e. a long lifetime and a high quantum yield) for photoinduced charge separation. The targeted delivery of 1-Oct to mitochondria was accomplished by using a combination of the Fc-ZnP-C60 molecule and a drug delivery nano-carrier, MITO-Porter, that was recently established by our group for intracellular cargo delivery. The successful delivery of 1-Oct by the MITO-Porter permitted the optically-controlled generation of O2 in the mitochondria of HeLa cells and the following induction of apoptosis as a cell signalling response was observed in confocal laser microscopy experiments. The obtained results indicate the use of an electron donor–acceptor system such as this can be a promising tool for the non-invasive triggering of redox-coupled cellular activities in living systems.

Graphical abstract: Optical control of mitochondrial reductive reactions in living cells using an electron donor–acceptor linked molecule

Supplementary files

Article information


Submitted
24 Aug 2017
Accepted
02 Nov 2017
First published
22 Nov 2017

Nanoscale, 2017,9, 18690-18698
Article type
Paper

Optical control of mitochondrial reductive reactions in living cells using an electron donor–acceptor linked molecule

Y. Takano, R. Munechika, V. Biju, H. Harashima, H. Imahori and Y. Yamada, Nanoscale, 2017, 9, 18690 DOI: 10.1039/C7NR06310E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements