Jump to main content
Jump to site search

Issue 17, 2017

Development and validation of green chromatography for the determination of anthocyanins in haskap berry, mulberry and blackberry

Author affiliations

Abstract

This study was aimed at developing and validating a green HPLC-DAD method to determine anthocyanins using ethanol and an alpha-hydroxy acid aqueous solution as a mobile phase. The effects of alpha-hydroxy acids on the chromatographic behaviors of cyanidin-3-O-glucoside, the most predominant anthocyanin in nature, in a C18 column were investigated. Among seven selected alpha-hydroxy acids, tartaric acid exhibited potential for improving the chromatographic separation of cyanidin-3-O-glucoside. Cyanidin-3-O-glucoside was the main anthocyanin in haskap berry, mulberry, and blackberry, and the three berries were selected for further method development and validation. The optimized mobile phase was a mixture of ethanol and a 0.25 mol L−1 tartaric acid aqueous solution at the 20 : 80 (v/v) ratio. The retention time of cyanidin-3-O-glucoside was less than 1.8 min, and an optimum separation of anthocyanins was achieved in 4 min. The developed method was validated in terms of linearity, limits of detection and quantification, precision, repeatability, stability, and recovery. The calibration curve revealed good linearity (R2 = 0.9999), and the limits of detection and quantification were 0.038 mg L−1 and 0.125 mg L−1, respectively. The relative standard deviations of intra- and inter-day precision, repeatability, and stability were less than 5%. The recoveries of cyanidin-3-O-glucoside from the berry samples were between 99% and 101%, a satisfactory level. Additionally, the optimized mobile phase had a negligible effect on the C18 column stability. Therefore, the developed method was safe, rapid, and valid for anthocyanin analysis.

Graphical abstract: Development and validation of green chromatography for the determination of anthocyanins in haskap berry, mulberry and blackberry

Supplementary files

Article information


Submitted
24 Jan 2017
Accepted
03 Apr 2017
First published
05 Apr 2017

Anal. Methods, 2017,9, 2535-2545
Article type
Paper

Development and validation of green chromatography for the determination of anthocyanins in haskap berry, mulberry and blackberry

J. Sang, Q. Ma and C. Li, Anal. Methods, 2017, 9, 2535 DOI: 10.1039/C7AY00229G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements