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Issue 17, 2017
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Competitive multicomponent anion exchange adsorption of proteins at the single molecule level

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Abstract

We use single molecule spectroscopy to study a multicomponent, competitive protein adsorption system. Fluorescently-labeled α-lactalbumin proteins are super-resolved adsorbing to cationic anion-exchange ligands in the presence of a competitor, insulin. We find that the competitor reduces the number of binding events by blocking ligands throughout the observed measurement time while the single-site adsorption kinetics are unchanged.

Graphical abstract: Competitive multicomponent anion exchange adsorption of proteins at the single molecule level

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Article information


Submitted
27 Apr 2017
Accepted
14 Jul 2017
First published
18 Jul 2017

Analyst, 2017,142, 3127-3131
Article type
Communication

Competitive multicomponent anion exchange adsorption of proteins at the single molecule level

L. Kisley, U. Patil, S. Dhamane, K. Kourentzi, L. J. Tauzin, R. C. Willson and C. F. Landes, Analyst, 2017, 142, 3127
DOI: 10.1039/C7AN00701A

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