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Issue 12, 2017
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Adsorption of the natural protein surfactant Rsn-2 onto liquid interfaces

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To stabilize foams, droplets and films at liquid interfaces a range of protein biosurfactants have evolved in nature. Compared to synthetic surfactants, these combine surface activity with biocompatibility and low solution aggregation. One recently studied example is Rsn-2, a component of the foam nest of the frog Engystomops pustulosus, which has been predicted to undergo a clamshell-like opening transition at the air–water interface. Using atomistic molecular dynamics simulations and surface tension measurements we study the adsorption of Rsn-2 onto air–water and cyclohexane–water interfaces. The protein adsorbs readily at both interfaces, with adsorption mediated by the hydrophobic N-terminus. At the cyclohexane–water interface the clamshell opens, due to the favourable interaction between hydrophobic residues and cyclohexane molecules and the penetration of cyclohexane molecules into the protein core. Simulations of deletion mutants showed that removal of the N-terminus inhibits interfacial adsorption, which is consistent with the surface tension measurements. Deletion of the hydrophilic C-terminus also affects adsorption, suggesting that this plays a role in orienting the protein at the interface. The characterisation of the interfacial behaviour gives insight into the factors that control the interfacial adsorption of proteins, which may inform new applications of this and similar proteins in areas including drug delivery and food technology and may also be used in the design of synthetic molecules showing similar changes in conformation at interfaces.

Graphical abstract: Adsorption of the natural protein surfactant Rsn-2 onto liquid interfaces

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Publication details

The article was received on 23 Oct 2016, accepted on 27 Feb 2017 and first published on 27 Feb 2017

Article type: Paper
DOI: 10.1039/C6CP07261E
Phys. Chem. Chem. Phys., 2017,19, 8584-8594

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    Adsorption of the natural protein surfactant Rsn-2 onto liquid interfaces

    G. B. Brandani, S. J. Vance, M. Schor, A. Cooper, M. W. Kennedy, B. O. Smith, C. E. MacPhee and D. L. Cheung, Phys. Chem. Chem. Phys., 2017, 19, 8584
    DOI: 10.1039/C6CP07261E

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