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Issue 39, 2016
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Probing cell internalisation mechanics with polymer capsules

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Abstract

We report polymer capsule-based probes for quantifying the pressure exerted by cells during capsule internalisation (Pin). Poly(methacrylic acid) (PMA) capsules with tuneable mechanical properties were fabricated through layer-by-layer assembly. The Pin was quantified by correlating the cell-induced deformation with the ex situ osmotically induced deformation of the polymer capsules. Ultimately, we found that human monocyte-derived macrophage THP-1 cells exerted up to approximately 360 kPa on the capsules during internalisation.

Graphical abstract: Probing cell internalisation mechanics with polymer capsules

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Publication details

The article was received on 22 Aug 2016, accepted on 11 Sep 2016 and first published on 22 Sep 2016


Article type: Communication
DOI: 10.1039/C6NR06657G
Citation: Nanoscale, 2016,8, 17096-17101
  • Open access: Creative Commons BY license
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    Probing cell internalisation mechanics with polymer capsules

    X. Chen, J. Cui, Y. Ping, T. Suma, F. Cavalieri, Q. A. Besford, G. Chen, J. A. Braunger and F. Caruso, Nanoscale, 2016, 8, 17096
    DOI: 10.1039/C6NR06657G

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