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Issue 10, 2016
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How flexible is a protein: simple estimates using FRET microscopy

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Abstract

Flexible proteins are frequently used to link subunits of larger complexes in various contexts, for instance, in the construction of unimolecular sensors used in FRET microscopy, and fusion proteins. How flexible such linkers are can be an important question in the overall design of the complex, and yet sometimes suprisingly difficult to establish. Such difficulties can arise because the actual flexibility of a protein depends significantly on its interactions with the solvent, and when the local environment is a subcellular compartment, even the conditions of the solvent, may not be known. In this communication we propose a simple numerical procedure through which the flexibility of such proteins can be extracted from FRET based microscopy data.

Graphical abstract: How flexible is a protein: simple estimates using FRET microscopy

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Publication details

The article was received on 24 May 2016, accepted on 02 Aug 2016 and first published on 02 Aug 2016


Article type: Communication
DOI: 10.1039/C6MB00410E
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Citation: Mol. BioSyst., 2016,12, 2988-2991

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    How flexible is a protein: simple estimates using FRET microscopy

    S. Sanyal, D. F. Coker and D. MacKernan, Mol. BioSyst., 2016, 12, 2988
    DOI: 10.1039/C6MB00410E

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