Issue 20, 2016

Polymerase chain reaction in microfluidic devices


The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules across several orders of magnitude. Since the first application of PCR in a microfluidic device was developed in 1998, an increasing number of researchers have continued the development of microfluidic PCR systems. In this review, we introduce recent developments in microfluidic-based space and time domain devices as well as discuss various designs integrated with multiple functions for sample preparation and detection. The development of isothermal nucleic acid amplification and digital PCR microfluidic devices within the last five years is also highlighted. Furthermore, we introduce various commercial microfluidic PCR devices.

Graphical abstract: Polymerase chain reaction in microfluidic devices

Article information

Article type
Critical Review
02 Aug 2016
06 Sep 2016
First published
06 Sep 2016
This article is Open Access
Creative Commons BY license

Lab Chip, 2016,16, 3866-3884

Polymerase chain reaction in microfluidic devices

C. D. Ahrberg, A. Manz and B. G. Chung, Lab Chip, 2016, 16, 3866 DOI: 10.1039/C6LC00984K

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity