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Issue 11, 2016
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Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples

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Abstract

Despite recent advances in microfluidic-based integrated diagnostic systems, the sample introduction interface, especially with regards to large volume samples, has often been neglected. We present a sample introduction interface that allows direct on-chip processing of crude stool samples for the detection of Helicobacter pylori (H. pylori). The principle of IFAST (immiscible filtration assisted by surface tension) was adapted to include a large volume sample chamber with a septum-based interface for stool sample introduction. Solid chaotropic salt and dry superparamagnetic particles (PMPs) could be stored on-chip and reconstituted upon sample addition, simplifying the process of release of DNA from H. pylori cells and its binding to the PMPs. Finally, the PMPs were pulled via a magnet through a washing chamber containing an immiscible oil solution and into an elution chamber where the DNA was released into aqueous media for subsequent analysis. The entire process required only 7 min while enabling a 40-fold reduction in working volume from crude biological samples. The combination of a real-world interface and rapid DNA extraction offers the potential for the methodology to be used in point-of-care (POC) devices.

Graphical abstract: Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples

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Publication details

The article was received on 18 Feb 2016, accepted on 28 Apr 2016 and first published on 10 May 2016


Article type: Paper
DOI: 10.1039/C6LC00228E
Citation: Lab Chip, 2016,16, 2108-2115
  • Open access: Creative Commons BY license
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    Sample introduction interface for on-chip nucleic acid-based analysis of Helicobacter pylori from stool samples

    O. Mosley, L. Melling, M. D. Tarn, C. Kemp, M. M. N. Esfahani, N. Pamme and K. J. Shaw, Lab Chip, 2016, 16, 2108
    DOI: 10.1039/C6LC00228E

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