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Issue 24, 2016
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Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

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Abstract

We describe organelle thermometry using an endoplasmic reticulum-targeting small molecule dye and cytosolic mCherry, whose fluorescence lifetimes reduce with increasing temperature and can be monitored by fluorescence lifetime imaging microscopy. The results show that heat production in single myotubes is highly localized and is coupled to a Ca2+ burst.

Graphical abstract: Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

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Supplementary files

Article information


Submitted
03 Dec 2015
Accepted
21 Jan 2016
First published
21 Jan 2016

This article is Open Access

Chem. Commun., 2016,52, 4458-4461
Article type
Communication
Author version available

Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

H. Itoh, S. Arai, T. Sudhaharan, S. Lee, Y. Chang, S. Ishiwata, M. Suzuki and E. B. Lane, Chem. Commun., 2016, 52, 4458
DOI: 10.1039/C5CC09943A

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