Issue 2, 2016
From the journal:

Chemical Communications

Access to side-chain carbon information in deuterated solids under fast MAS through non-rotor-synchronized mixing†

Natalia Kulminskaya,‡a   Suresh Kumar Vasa,‡a   Karin Giller,a   Stefan Becker,a   Ann Kwan,b   Margaret Sundeb  and  Rasmus Linser*a  

* Corresponding authors

a Max-Planck Institute for Biophysical Chemistry, Department NMR-Based Structural Biology, Am Fassberg 11, 37077 Göttingen, Germany
E-mail: rali@nmr.mpibpc.mpg.de

b School of Medical Sciences and School of Molecular Bioscience, University of Sydney, Sydney, Australia

Abstract

We demonstrate the accessibility of aliphatic 13C side chain chemical shift sets for solid-state NMR despite perdeuteration and fast MAS using isotropic, non-rotor-synchronized 13C–13C mixing. Combined with amide proton detection, we unambiguously and sensitively detect whole side chain to backbone correlations for two proteins using around 1 mg of sample.

Graphical abstract: Access to side-chain carbon information in deuterated solids under fast MAS through non-rotor-synchronized mixing

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C5CC07345F
Article type
Communication
Submitted
01 Sep 2015
Accepted
21 Oct 2015
First published
22 Oct 2015

Chem. Commun., 2016,52, 268-271

Author version available

Download author version (PDF)

Permissions

Request permissions

Access to side-chain carbon information in deuterated solids under fast MAS through non-rotor-synchronized mixing

N. Kulminskaya, S. K. Vasa, K. Giller, S. Becker, A. Kwan, M. Sunde and R. Linser, Chem. Commun., 2016, 52, 268 DOI: 10.1039/C5CC07345F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements