Jump to main content
Jump to site search

Issue 1, 2016
Previous Article Next Article

New insights into the complex regulation of the glycolytic pathway in Lactococcus lactis. I. Construction and diagnosis of a comprehensive dynamic model

Author affiliations

Abstract

This article and the companion paper use computational systems modeling to decipher the complex coordination of regulatory signals controlling the glycolytic pathway in the dairy bacterium Lactococcus lactis. In this first article, the development of a comprehensive kinetic dynamic model is described. The model is based on in vivo NMR data that consist of concentration trends in key glycolytic metabolites and cofactors. The model structure and parameter values are identified with a customized optimization strategy that uses as its core the method of dynamic flux estimation. For the first time, a dynamic model with a single parameter set fits all available glycolytic time course data under anaerobic operation. The model captures observations that had not been addressed so far and suggests the existence of regulatory effects that had been observed in other species, but not in L. lactis. The companion paper uses this model to analyze details of the dynamic control of glycolysis under aerobic and anaerobic conditions.

Graphical abstract: New insights into the complex regulation of the glycolytic pathway in Lactococcus lactis. I. Construction and diagnosis of a comprehensive dynamic model

Back to tab navigation

Article information


Submitted
12 May 2015
Accepted
04 Nov 2015
First published
06 Nov 2015

Mol. BioSyst., 2016,12, 23-36
Article type
Paper
Author version available

New insights into the complex regulation of the glycolytic pathway in Lactococcus lactis. I. Construction and diagnosis of a comprehensive dynamic model

S. Dolatshahi, L. L. Fonseca and E. O. Voit, Mol. BioSyst., 2016, 12, 23
DOI: 10.1039/C5MB00331H

Search articles by author

Spotlight

Advertisements