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Issue 1, 2016
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A portable optical waveguide resonance light-scattering scanner for microarray detection

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Abstract

In the present work, a portable and low-cost planar waveguide based resonance light scattering (RLS) scanner (termed as: PW-RLS scanner) has been developed for microarray detection. The PW-RLS scanner employs a 2 × 4 white light emitting diode array (WLEDA) as the excitation light source, a folded optical path with a complementary metal oxide semiconductor (CMOS) as the signal/image acquisition device and stepper motors with gear drives as the mechanical drive system. The biological binding/recognizing events on the microarray can be detected with an evanescent waveguide-directed illumination and light-scattering label (e.g., nanoparticles) while the microarray slide acts as an evanescent waveguide substrate. The performance of the as-developed PW-RLS scanner has been evaluated by analyzing type 2 diabetes mellitus (T2DM) risk genes. Highly selective and sensitive (less than 1% allele frequency at the attomole-level) T2DM risk gene detection is achieved using single-stranded DNA functionalized gold nanoparticles (ssDNA-GNPs) as detection probes. Additionally, the successful simultaneous analysis of 15 T2DM patient genotypes suggests that the device has great potential for the realization of a personalized diagnostic test for a given disease or patient follow-up.

Graphical abstract: A portable optical waveguide resonance light-scattering scanner for microarray detection

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Publication details

The article was received on 07 Sep 2015, accepted on 30 Oct 2015 and first published on 30 Oct 2015


Article type: Paper
DOI: 10.1039/C5AN01839K
Analyst, 2016,141, 199-205

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    A portable optical waveguide resonance light-scattering scanner for microarray detection

    X. Xing, W. Liu, T. Li, S. Xing, X. Fu, D. Wu, D. Liu and Z. Wang, Analyst, 2016, 141, 199
    DOI: 10.1039/C5AN01839K

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