Jump to main content
Jump to site search

Issue 13, 2015
Previous Article Next Article

Structure and function of the silicifying peptide R5

Author affiliations

Abstract

The 19-mer synthetic peptide known as R5 has been used widely in studies of peptide-driven silica condensation. Despite this, the structure and function of R5 have not yet been fully characterized. Here, we present a systematic study of R5 silicification focusing on three key variables: the concentration of the peptide, the concentration of the silica precursor silicic acid, and the solution pH. Additionally, we present the first study of R5 secondary structure in the presence and absence of silicic acid and introduce one-dimensional and two-dimensional solution NMR to probe both structure and higher-order peptide aggregation. We find that R5-directed silicification is linear with regard to silicic acid and H+ but, unexpectedly, that silicification appears to be cooperative with respect to peptide concentration. We also find that R5 is a random coil ensemble at subsaturating silicic acid concentrations and does not spontaneously self-assemble to form discrete aggregates in solution. These data contradict a model that invokes the functional micellization of R5 and provide a framework for future studies with the R5 peptide.

Graphical abstract: Structure and function of the silicifying peptide R5

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Oct 2014, accepted on 10 Feb 2015 and first published on 12 Feb 2015


Article type: Paper
DOI: 10.1039/C4TB01679C
Author version
available:
Download author version (PDF)
J. Mater. Chem. B, 2015,3, 2607-2614
  • Open access: Creative Commons BY license
  •   Request permissions

    Structure and function of the silicifying peptide R5

    L. Senior, M. P. Crump, C. Williams, P. J. Booth, S. Mann, A. W. Perriman and P. Curnow, J. Mater. Chem. B, 2015, 3, 2607
    DOI: 10.1039/C4TB01679C

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements