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Issue 5, 2015

Highly stable and reusable imprinted artificial antibody used for in situ detection and disinfection of pathogens

Author affiliations

Abstract

Sandwich ELISA methods have been widely used for biomarker and pathogen detection because of their high specificity and sensitivity. However, the main drawbacks of this assay are the cost, the time-consuming procedure for the isolation of antibodies and their poor stability. To overcome these restrictions, we herein fabricated artificial antibodies based on imprinting technology and developed a sandwich ELISA for pathogen detection. Both the capture and detection antibodies were obtained via an in situ method, with simplicity, rapidity and low cost. The peroxidase mimics, the CeO2 nanoparticles, as signal generators were integrated with the detection antibody. The fabricated artificial antibodies exhibited not only natural antibody-like binding affinities and selectivities, but also superior stability and reusability. The detection limit was about 500 CFU mL−1, which is much lower than that of traditional ELISA methods (104 to 105 CFU mL−1). Furthermore, the capture antibody can disinfect pathogens in situ.

Graphical abstract: Highly stable and reusable imprinted artificial antibody used for in situ detection and disinfection of pathogens

Supplementary files

Article information


Submitted
09 Feb 2015
Accepted
15 Feb 2015
First published
23 Feb 2015

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2015,6, 2822-2826
Article type
Edge Article

Highly stable and reusable imprinted artificial antibody used for in situ detection and disinfection of pathogens

Z. Zhang, Y. Guan, M. Li, A. Zhao, J. Ren and X. Qu, Chem. Sci., 2015, 6, 2822 DOI: 10.1039/C5SC00489F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.


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