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Issue 5, 2015

Ratiometric detection of pH fluctuation in mitochondria with a new fluorescein/cyanine hybrid sensor

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Abstract

The homeostasis of mitochondrial pH (pHm) is crucial in cell physiology. Developing small-molecular fluorescent sensors for the ratiometric detection of pHm fluctuation is highly demanded yet challenging. A ratiometric pH sensor, Mito-pH, was constructed by integrating a pH-sensitive FITC fluorophore with a pH-insensitive hemicyanine group. The hemicyanine group also acts as the mitochondria targeting group due to its lipophilic cationic nature. Besides its ability to target mitochondria, this sensor provides two ratiometric pH sensing modes, the dual excitation/dual emission mode (Dex/Dem) and dual excitation (Dex) mode, and its linear and reversible ratiometric response range from pH 6.15 to 8.38 makes this sensor suitable for the practical tracking of pHm fluctuation in live cells. With this sensor, stimulated pHm fluctuation has been successfully tracked in a ratiometric manner via both fluorescence imaging and flow cytometry.

Graphical abstract: Ratiometric detection of pH fluctuation in mitochondria with a new fluorescein/cyanine hybrid sensor

Supplementary files

Article information


Submitted
28 Dec 2014
Accepted
16 Mar 2015
First published
16 Mar 2015

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2015,6, 3187-3194
Article type
Edge Article
Author version available

Ratiometric detection of pH fluctuation in mitochondria with a new fluorescein/cyanine hybrid sensor

Y. Chen, C. Zhu, J. Cen, Y. Bai, W. He and Z. Guo, Chem. Sci., 2015, 6, 3187 DOI: 10.1039/C4SC04021J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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