Issue 7, 2015

Charge-selective membrane protein patterning with proteoliposomes

Abstract

A novel method to fabricate transmembrane protein (TP) embedded lipid bilayers using microcontact printing and applying proteoliposomes to different types of substrates, has been developed. The electrostatic interaction between the negatively charged proteoliposome and the substrate, which had been positively functionalized by microcontact printing, allowed the formation of TP-embedded, patterned lipid bilayers. The positively charged amino functional group on the substrate did effectively attract the negatively charged vesicles, inducing them to be adsorbed and subsequently ruptured to form a giant mosaic lipid bilayer, resulting in an immobilized TP-embedded lipid layer precisely on the targeted patterns, which were backfilled with a zwitterionic lipid bilayer. The rapid and highly selective recognition of the charged liposomes was visualized, and the biological functions of the TPs in the lipid matrix were also observed.

Graphical abstract: Charge-selective membrane protein patterning with proteoliposomes

Supplementary files

Article information

Article type
Paper
Submitted
09 Oct 2014
Accepted
05 Dec 2014
First published
09 Dec 2014

RSC Adv., 2015,5, 5183-5191

Author version available

Charge-selective membrane protein patterning with proteoliposomes

H. Kim, K. Y. Lee, S. R. Ryu, K. Jung, T. K. Ahn, Y. Lee, O. Kwon, S. Park, K. K. Parker and K. Shin, RSC Adv., 2015, 5, 5183 DOI: 10.1039/C4RA12088D

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