Jump to main content
Jump to site search

Issue 9, 2015
Previous Article Next Article

Characterizing rhodopsin signaling by EPR spectroscopy: from structure to dynamics

Author affiliations

Abstract

Electron paramagnetic resonance (EPR) spectroscopy, together with spin labeling techniques, has played a major role in the characterization of rhodopsin, the photoreceptor protein and G protein-coupled receptor (GPCR) in rod cells. Two decades ago, these biophysical tools were the first to identify transmembrane helical movements in rhodopsin upon photo-activation, a critical step in the study of GPCR signaling. EPR methods were employed to identify functional loop dynamics within rhodopsin, to measure light-induced millisecond timescale changes in rhodopsin conformation, to characterize the effects of partial agonists on the apoprotein opsin, and to study lipid interactions with rhodopsin. With the emergence of advanced pulsed EPR techniques, the stage was set to determine the amplitude of structural changes in rhodopsin and the dynamics in the rhodopsin signaling complexes. Work in this area has yielded invaluable information about mechanistic properties of GPCRs. Using EPR techniques, receptors are studied in native-like membrane environments and the effects of lipids on conformational equilibria can be explored. This perspective addresses the impact of EPR methods on rhodopsin and GPCR structural biology, highlighting historical discoveries made with spin labeling techniques, and outlining exciting new directions in the field.

Graphical abstract: Characterizing rhodopsin signaling by EPR spectroscopy: from structure to dynamics

Back to tab navigation

Article information


Submitted
04 May 2015
Accepted
22 Jun 2015
First published
23 Jun 2015

Photochem. Photobiol. Sci., 2015,14, 1586-1597
Article type
Perspective
Author version available

Characterizing rhodopsin signaling by EPR spectroscopy: from structure to dynamics

N. Van Eps, L. N. Caro, T. Morizumi and O. P. Ernst, Photochem. Photobiol. Sci., 2015, 14, 1586 DOI: 10.1039/C5PP00191A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Search articles by author

Spotlight

Advertisements