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Issue 81, 2015
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Bulged Invader probes: activated duplexes for mixed-sequence dsDNA recognition with improved thermodynamic and kinetic profiles

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Abstract

Double-stranded oligonucleotides with +1 interstrand zipper arrangements of intercalator-functionalized nucleotides are energetically activated for recognition of mixed-sequence double-stranded DNA. Incorporation of nonyl (C9) bulges at specific positions of these probes, results in more highly affine (>5-fold), faster (>4-fold) and more persistent dsDNA recognition relative to conventional Invader probes.

Graphical abstract: Bulged Invader probes: activated duplexes for mixed-sequence dsDNA recognition with improved thermodynamic and kinetic profiles

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Publication details

The article was received on 28 Jul 2015, accepted on 14 Aug 2015 and first published on 18 Aug 2015


Article type: Communication
DOI: 10.1039/C5CC06264K
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Chem. Commun., 2015,51, 15051-15054

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    Bulged Invader probes: activated duplexes for mixed-sequence dsDNA recognition with improved thermodynamic and kinetic profiles

    D. C. Guenther, S. Karmakar and P. J. Hrdlicka, Chem. Commun., 2015, 51, 15051
    DOI: 10.1039/C5CC06264K

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