Issue 84, 2015
From the journal:

Chemical Communications

A two-photon activatable amino acid linker for the induction of fluorescence†

Felix Friedrich,a   Kathrin Klehs,b   Manuela A. H. Fichte,a   Stephan Junek,c   Mike Heilemann*b  and  Alexander Heckel*a  

* Corresponding authors

a Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany
E-mail: heckel@uni-frankfurt.de

b Goethe University Frankfurt, Institute for Physical and Theoretical Chemistry, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany
E-mail: heilemann@chemie.uni-frankfurt.de

c Imaging facility, Max Planck Institute for Brain Research, Max-von-Laue-Straße 4, 60438 Frankfurt am Main, Germany

Abstract

A new one- and two-photon activatable fluorophore based on ATTO565 was developed using a photolabile linker that simultaneously acts as a quencher. It is especially interesting for protein and peptide applications because it can be incorporated by standard peptide chemistry. The application of the new fluorogenic construct in super-resolution microscopy of antibody conjugates is shown.

Graphical abstract: A two-photon activatable amino acid linker for the induction of fluorescence

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Article information

DOI
https://doi.org/10.1039/C5CC05700K
Article type
Communication
Submitted
09 Jul 2015
Accepted
21 Aug 2015
First published
24 Aug 2015
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 15382-15385

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A two-photon activatable amino acid linker for the induction of fluorescence

F. Friedrich, K. Klehs, M. A. H. Fichte, S. Junek, M. Heilemann and A. Heckel, Chem. Commun., 2015, 51, 15382 DOI: 10.1039/C5CC05700K

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