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Issue 23, 2015
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Rapid label-free detection of E. coli using antimicrobial peptide assisted impedance spectroscopy

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Abstract

There is an increasing demand for rapid detection of waterborne pathogens to monitor drinking water safety. We demonstrate a compact, label-free sensor array for rapid detection of Escherichia coli (E. coli) in contaminated water samples using antimicrobial peptide assisted impedimetric sensor platform. Interdigitated electrode arrays immobilized with the antimicrobial peptide Colicin V (ColV) were used to screen the affinity towards different bacterial strains by monitoring impedance variations in real-time. This ColV assisted impedance biosensor exhibited high selectivity towards Gram-negative strains particularly towards E. coli strains. This selective detection of E. coli from other strains was observed at 102 cfu mL−1, which is clinically relevant. The sensor can detect E. coli from 102 to 106 cfu mL−1 in water sample at pH 7 to 9. These results show that the antimicrobial peptide ColV assisted impedimetric array is capable of rapid, specific detection of E. coli in contaminated water samples.

Graphical abstract: Rapid label-free detection of E. coli using antimicrobial peptide assisted impedance spectroscopy

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Article information


Submitted
22 Jul 2015
Accepted
22 Aug 2015
First published
25 Aug 2015

Anal. Methods, 2015,7, 9744-9748
Article type
Communication
Author version available

Rapid label-free detection of E. coli using antimicrobial peptide assisted impedance spectroscopy

K. Jiang, H. Etayash, S. Azmi, S. Naicker, M. Hassanpourfard, P. M. Shaibani, G. Thakur, K. Kaur and T. Thundat, Anal. Methods, 2015, 7, 9744
DOI: 10.1039/C5AY01917F

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