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Issue 18, 2015
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Colorimetric detection of penicillin antibiotic residues in pork using hybrid magnetic nanoparticles and penicillin class-selective, antibody-functionalized platinum nanoparticles

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Abstract

Here we describe the development of a rapid and facile method for the colorimetric detection of penicillin antibiotics, including penicillin G, penicillin V, nafcillin, oxacillin, cloxacillin and dicloxacillin, using hybrid magnetic nanoparticle clusters (HMNCs) and penicillin class-selective, antibody-functionalized platinum nanoparticles (Ab-Pt). Amine-functionalized HMNCs were used to chemically capture antibiotics from sample solution through the formation of amide bonds between amine groups of HMNCs and carboxyl groups of the antibiotics. Ab-Pt was then allowed to bind to antibiotic–HMNC complexes, after which the resulting Pt–antibiotic–HMNCs were added to a 3,3′,5,5′-tetramethylbenzidine (TMB) solution. The color change caused by platinum nanoparticle-mediated oxidation of TMB enabled antibiotics to be identified with the naked eye and quantified by measuring light absorption. The use of the penicillin class-selective antibody allowed the simultaneous detection of penicillin class antibiotics, thereby reducing the cost, time, and effort associated with prescreening antibiotic residues in sample specimens.

Graphical abstract: Colorimetric detection of penicillin antibiotic residues in pork using hybrid magnetic nanoparticles and penicillin class-selective, antibody-functionalized platinum nanoparticles

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Supplementary files

Article information


Submitted
20 Jun 2015
Accepted
25 Jul 2015
First published
27 Jul 2015

Anal. Methods, 2015,7, 7639-7645
Article type
Paper

Colorimetric detection of penicillin antibiotic residues in pork using hybrid magnetic nanoparticles and penicillin class-selective, antibody-functionalized platinum nanoparticles

D. Kwon, W. Lee, W. Kim, H. Yoo, H. Shin and S. Jeon, Anal. Methods, 2015, 7, 7639
DOI: 10.1039/C5AY01590A

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