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Issue 18, 2015
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Highly sensitive and selective coumarin probe for hydrogen sulfide imaging in living cells

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Abstract

Hydrogen sulfide (H2S) is the third endogenous gasotransmitter, following nitric oxide (NO) and carbon monoxide (CO). Efficient methods for selective monitoring H2S in biological systems are significant. In this study, we designed and synthesized a new coumarin-based probe DNPOCA for H2S with colorimetric and fluorescent dual signaling. In this probe, 7-hydroxycoumarin-4-acetic acid methyl ester was employed as a fluorophore and a dinitrophenyl (DNP) ether moiety was used as the recognition unit. The H2S probe exhibited excellent selectivity and high sensitivity with a detection limit as low as 49.7 nM. The cytotoxicity assay showed that DNPOCA possessed a low effect on cell viability. Furthermore, DNPOCA could be successfully applied for H2S imaging in living cells.

Graphical abstract: Highly sensitive and selective coumarin probe for hydrogen sulfide imaging in living cells

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Supplementary files

Article information


Submitted
24 Jun 2015
Accepted
28 Jul 2015
First published
29 Jul 2015

Anal. Methods, 2015,7, 7646-7652
Article type
Paper
Author version available

Highly sensitive and selective coumarin probe for hydrogen sulfide imaging in living cells

S. Chen, C. Ma, D. Wang, X. Han, L. Zhang and J. Wang, Anal. Methods, 2015, 7, 7646
DOI: 10.1039/C5AY01624J

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