Issue 5, 2015
From the journal:

Analyst

Comparative analysis of monoclonal antibody N-glycosylation using stable isotope labelling and UPLC-fluorescence-MS†

Silvia Millán Martín,a   Cédric Delporte,ac   Amy Farrell,a   Natalia Navas Iglesias,b   Niaobh McLoughlina  and  Jonathan Bones*a  

* Corresponding authors

a Characterisation and Comparability Laboratory, NIBRT – The National Institute for Bioprocessing Research and Training, Foster Avenue, Mount Merrion, Blackrock, Co., Dublin, Ireland
E-mail: jonathan.bones@nibrt.ie
Tel: +353 1215 8105

b Department of Analytical Chemistry, Faculty of Science, Biomedical Research Institute, University of Granada, Avenue Fuentenueva S/N, 18071 Granada, Spain

c Laboratory of Pharmaceutical Chemistry & Analytical Platform of the Faculty of Pharmacy, Université Libre de Bruxelles, Bld. du Triomphe, Campus Plaine, CP 205/05, B-1050 Brussels, Belgium

Abstract

A twoplex method using 12C6 and 13C6 stable isotope analogues (Δmass = 6 Da) of 2-aminobenzoic acid (2-AA) is described for quantitative analysis of N-glycans present on monoclonal antibodies and other glycoproteins using ultra performance liquid chromatography with sequential fluorescence and accurate mass tandem quadrupole time of flight (QToF) mass spectrometric detection.

Graphical abstract: Comparative analysis of monoclonal antibody N-glycosylation using stable isotope labelling and UPLC-fluorescence-MS

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Article information

DOI
https://doi.org/10.1039/C4AN02345E
Article type
Communication
Submitted
19 Dec 2014
Accepted
15 Jan 2015
First published
15 Jan 2015

Analyst, 2015,140, 1442-1447

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Comparative analysis of monoclonal antibody N-glycosylation using stable isotope labelling and UPLC-fluorescence-MS

S. Millán Martín, C. Delporte, A. Farrell, N. Navas Iglesias, N. McLoughlin and J. Bones, Analyst, 2015, 140, 1442 DOI: 10.1039/C4AN02345E

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