A novel and advanced Fc-binding probe – FcUni-RLuc namely – has been produced and functionally assayed for labelling IgGs. The Fc antibody binding sequence – HWRGWV – was fused to Renilla luciferase, and the purified probe was employed for bioluminescence enzyme-linked immunoabsorbance assay of Her2 positive cells.
A. Farzannia, R. Roghanian, S. H. Zarkesh-Esfahani, M. Nazari and R. Emamzadeh, Analyst, 2015, 140, 1438 DOI: 10.1039/C4AN01946F
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