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Issue 3, 2015
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Probing the secondary structure of bovine serum albumin during heat-induced denaturation using mid-infrared fiberoptic sensors

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Abstract

Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy using a special waveguide based on a silver halide fiber was used for probing the heat-induced secondary structure and conformation changes of bovine serum albumin (BSA). From the secondary derivative and the curve fitting of the obtained ATR-FTIR spectra, the changes of the BSA secondary structure with temperature were clearly identified. Two different thermal denaturation temperature ranges (i.e., 50–52 and 80–82 °C, at which a change of the protein structure occurred) were determined, while only one denaturation temperature was previously identified via classical FTIR measurements. Additionally, taking advantage of two-dimensional correlation spectroscopy more detailed information on changes of the protein secondary structure was revealed. The developed method facilitates in situ, sensitive, and more in-depth probing of protein secondary structures, which represents a significant advancement compared to conventional characterization methods.

Graphical abstract: Probing the secondary structure of bovine serum albumin during heat-induced denaturation using mid-infrared fiberoptic sensors

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Publication details

The article was received on 14 Aug 2014, accepted on 11 Nov 2014 and first published on 12 Nov 2014


Article type: Paper
DOI: 10.1039/C4AN01495B
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Analyst, 2015,140, 765-770
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    Probing the secondary structure of bovine serum albumin during heat-induced denaturation using mid-infrared fiberoptic sensors

    R. Lu, W. Li, A. Katzir, Y. Raichlin, H. Yu and B. Mizaikoff, Analyst, 2015, 140, 765
    DOI: 10.1039/C4AN01495B

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