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Issue 15, 2015

Self-assembling dual component nanoparticles with endosomal escape capability

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This study reports a novel nanoparticle system with simple and modular one-step assembly, which can respond intelligently to biologically relevant variations in pH. Importantly, these particles also show the ability to induce escape from the endosomal/lysosomal compartments of the cell, which is integral to the design of efficient polymeric delivery systems. The nanoparticles were formed by the nanoprecipitation of pH-responsive poly(2-(diethylamino)ethyl methacrylate) (PDEAEMA) and poly(2-(diethylamino)ethyl methacrylate)-b-poly(ethylene glycol) (PDEAEMA-b-PEG). Rhodamine B octadecyl ester perchlorate was successfully encapsulated within the hydrophobic core of the nanoparticle upon nanoprecipitation into PBS at pH 8. These particles disassembled when the pH was reduced below 6.8 at 37 °C. Cellular experiments showed the successful uptake of the nanoparticles into the endosomal/lysosomal compartments of 3T3 fibroblast cells. The ability to induce escape from the endosomes was demonstrated by the use of calcein, a membrane-impermeable fluorophore. The modular nature of these particles combined with promising endosomal escape capabilities make these dual component PDEAEMA nanoparticles useful for drug and gene delivery applications.

Graphical abstract: Self-assembling dual component nanoparticles with endosomal escape capability

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Article information

12 Jan 2015
23 Feb 2015
First published
24 Feb 2015

This article is Open Access

Soft Matter, 2015,11, 2993-3002
Article type
Author version available

Self-assembling dual component nanoparticles with endosomal escape capability

A. S. M. Wong, S. K. Mann, E. Czuba, A. Sahut, H. Liu, T. C. Suekama, T. Bickerton, A. P. R. Johnston and G. K. Such, Soft Matter, 2015, 11, 2993 DOI: 10.1039/C5SM00082C

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