Issue 1, 2015
From the journal:

Chemical Science

Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA†

Anika Kerna  and  Oliver Seitz*a  

* Corresponding authors

a Institut für Chemie, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489 Berlin, Germany
E-mail: oliver.seitz@chemie.hu-berlin.de
Fax: +49-30-2093-7266

Abstract

Several genomic disorders are caused by an excessive number of DNA triplet repeats. We developed a DNA-templated reaction in which product formation occurs only when the number of repeats exceeds a threshold indicative for the outbreak of Chorea Huntington. The combined use of native chemical PNA ligation and auxiliary DNA probes enabled reactions on templates obtained from human genomic DNA.

Graphical abstract: Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA

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Article information

DOI
https://doi.org/10.1039/C4SC01974A
Article type
Edge Article
Submitted
02 Jul 2014
Accepted
16 Sep 2014
First published
16 Sep 2014
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2015,6, 724-728

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Template-directed ligation on repetitive DNA sequences: a chemical method to probe the length of Huntington DNA

A. Kern and O. Seitz, Chem. Sci., 2015, 6, 724 DOI: 10.1039/C4SC01974A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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