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Issue 40, 2015
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Lactoferrin conjugated iron oxide nanoparticles for targeting brain glioma cells in magnetic particle imaging

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Abstract

Magnetic Particle Imaging (MPI) is a new real-time imaging modality, which promises high tracer mass sensitivity and spatial resolution directly generated from iron oxide nanoparticles. In this study, monodisperse iron oxide nanoparticles with median core diameters ranging from 14 to 26 nm were synthesized and their surface was conjugated with lactoferrin to convert them into brain glioma targeting agents. The conjugation was confirmed with the increase of the hydrodynamic diameters, change of zeta potential, and Bradford assay. Magnetic particle spectrometry (MPS), performed to evaluate the MPI performance of these nanoparticles, showed no change in signal after lactoferrin conjugation to nanoparticles for all core diameters, suggesting that the MPI signal is dominated by Néel relaxation and thus independent of hydrodynamic size difference or presence of coating molecules before and after conjugations. For this range of core sizes (14–26 nm), both MPS signal intensity and spatial resolution improved with increasing core diameter of nanoparticles. The lactoferrin conjugated iron oxide nanoparticles (Lf-IONPs) showed specific cellular internalization into C6 cells with a 5-fold increase in MPS signal compared to IONPs without lactoferrin, both after 24 h incubation. These results suggest that Lf-IONPs can be used as tracers for targeted brain glioma imaging using MPI.

Graphical abstract: Lactoferrin conjugated iron oxide nanoparticles for targeting brain glioma cells in magnetic particle imaging

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Article information


Submitted
30 Apr 2015
Accepted
13 Sep 2015
First published
22 Sep 2015

Nanoscale, 2015,7, 16890-16898
Article type
Paper
Author version available

Lactoferrin conjugated iron oxide nanoparticles for targeting brain glioma cells in magnetic particle imaging

A. Tomitaka, H. Arami, S. Gandhi and K. M. Krishnan, Nanoscale, 2015, 7, 16890
DOI: 10.1039/C5NR02831K

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