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Issue 11, 2015
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A novel glutathione-stabilized silver–gold nano-alloy/Cu2+ combination as a fluorescent switch probe for l-histidine

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Abstract

A new and environmentally friendly approach for the preparation of glutathione-stabilized silver–gold nano-alloys (GSH-AgAuNAs) with high fluorescence was proposed in this work. The as-prepared GSH-AgAuNAs were characterized with various methods and coupled with copper ions (Cu2+) to form a fluorescent switch probe (GSH-AgAuNAs/Cu2+ combination) for the detection of L-histidine. The fluorescence of the GSH-AgAuNAs was first quenched by adding an appropriate amount of Cu2+ solution. Then, in the presence of L-histidine, the GSH-AgAuNAs/Cu2+ combination solution exhibited an obvious fluorescence enhancement due to the specific interaction between the imidazole group of L-histidine and Cu2+. The strong chelation between L-histidine and Cu2+ illustrated the feasibility of constructing a selective “switch on” probe for the detection of L-histidine over other amino acids. Different from other methods for detecting L-histidine based on fluorescent nanomaterials, our work promises high selectivity, simplicity and the avoidance of organic solvents. Under the optimal conditions, the newly constructed GSH-AgAuNAs/Cu2+ fluorescent probe showed a satisfactory linear detection range of 2 to 40 μM, with a detection limit of 1.19 μM. The practical use of the GSH-AgAuNAs/Cu2+ combination in real human serum samples was tested, illustrating its potential application in L-histidine detection.

Graphical abstract: A novel glutathione-stabilized silver–gold nano-alloy/Cu2+ combination as a fluorescent switch probe for l-histidine

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Supplementary files

Article information


Submitted
12 Jul 2015
Accepted
21 Aug 2015
First published
24 Aug 2015

New J. Chem., 2015,39, 8569-8575
Article type
Paper
Author version available

A novel glutathione-stabilized silver–gold nano-alloy/Cu2+ combination as a fluorescent switch probe for L-histidine

X. Huang, Y. Lin, J. Chen, Y. Chen, Y. Li and W. Gao, New J. Chem., 2015, 39, 8569
DOI: 10.1039/C5NJ01819F

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