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A simple reaction procedure for chemiluminescence of firefly luciferin (D-luc) using n-propylphosphonic anhydride (T3P) is reported. A luminescent photon is produced as a result of one-pot reaction, only requiring mixing with the substrate carboxylic acid and T3P in the presence of a mild organic base.

Graphical abstract: A firefly inspired one-pot chemiluminescence system using n-propylphosphonic anhydride (T3P)

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