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Issue 8, 2014
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Dual-mode hydrodynamic railing and arraying of microparticles for multi-stage signal detection in continuous flow biochemical microprocessors

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Abstract

Continuous flow particulate-based microfluidic processors are in critical demand for emerging applications in chemistry and biology, such as point-of-care molecular diagnostics. Challenges remain, however, for accomplishing biochemical assays in which microparticle immobilization is desired or required during intermediate stages of fluidic reaction processes. Here we present a dual-mode microfluidic reactor that functions autonomously under continuous flow conditions to: (i) execute multi-stage particulate-based fluidic mixing routines, and (ii) array select numbers of microparticles during each reaction stage (e.g., for optical detection). We employ this methodology to detect the inflammatory cytokine, interferon-gamma (IFN-γ), via a six-stage aptamer-based sandwich assay.

Graphical abstract: Dual-mode hydrodynamic railing and arraying of microparticles for multi-stage signal detection in continuous flow biochemical microprocessors

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Supplementary files

Article information


Submitted
05 Jan 2014
Accepted
12 Feb 2014
First published
12 Feb 2014

Lab Chip, 2014,14, 1405-1409
Article type
Technical Innovation
Author version available

Dual-mode hydrodynamic railing and arraying of microparticles for multi-stage signal detection in continuous flow biochemical microprocessors

R. D. Sochol, D. Corbett, S. Hesse, W. E. R. Krieger, K. T. Wolf, M. Kim, K. Iwai, S. Li, L. P. Lee and L. Lin, Lab Chip, 2014, 14, 1405
DOI: 10.1039/C4LC00012A

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