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Issue 58, 2014
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Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine

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Abstract

This communication describes a general approach for site-specific fluorescence labelling of RNA using a cytidine triphosphate (CTP) analogue derivatized with a trans-cyclooctene group. The analogue was efficiently incorporated into a model RNA strand using in vitro transcription. Bio-orthogonal reaction with fluorescein-labelled tetrazine was utilized to fluorescently tag the synthetic RNA strand.

Graphical abstract: Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine

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Supplementary files

Article information


Submitted
02 Apr 2014
Accepted
02 Jun 2014
First published
03 Jun 2014

Chem. Commun., 2014,50, 7844-7847
Article type
Communication
Author version available

Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine

P. N. Asare-Okai, E. Agustin, D. Fabris and M. Royzen, Chem. Commun., 2014, 50, 7844
DOI: 10.1039/C4CC02435D

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