Jump to main content
Jump to site search

Issue 58, 2014
Previous Article Next Article

Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine

Author affiliations

Abstract

This communication describes a general approach for site-specific fluorescence labelling of RNA using a cytidine triphosphate (CTP) analogue derivatized with a trans-cyclooctene group. The analogue was efficiently incorporated into a model RNA strand using in vitro transcription. Bio-orthogonal reaction with fluorescein-labelled tetrazine was utilized to fluorescently tag the synthetic RNA strand.

Graphical abstract: Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine

Back to tab navigation

Supplementary files

Publication details

The article was received on 02 Apr 2014, accepted on 02 Jun 2014 and first published on 03 Jun 2014


Article type: Communication
DOI: 10.1039/C4CC02435D
Author version
available:
Download author version (PDF)
Citation: Chem. Commun., 2014,50, 7844-7847
  •   Request permissions

    Site-specific fluorescence labelling of RNA using bio-orthogonal reaction of trans-cyclooctene and tetrazine

    P. N. Asare-Okai, E. Agustin, D. Fabris and M. Royzen, Chem. Commun., 2014, 50, 7844
    DOI: 10.1039/C4CC02435D

Search articles by author

Spotlight

Advertisements