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Issue 38, 2014
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Influence of the Debye length on the interaction of a small molecule-modified Au nanoparticle with a surface-bound bioreceptor

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Abstract

We report that a shorter Debye length and, as a consequence, decreased colloidal stability are required for the molecular interaction of folic acid-modified Au nanoparticles (Au NPs) to occur on a surface-bound receptor, human dihydrofolate reductase (hDHFR). The interaction measured using surface plasmon resonance (SPR) sensing was optimal in a phosphate buffer at pH 6 and ionic strength exceeding 300 mM. Under these conditions, the aggregation constant of the Au NPs was approximately 104 M−1 s−1 and the Debye length was below 1 nm, on the same length scale as the size of the folate anion (approximately 0.8 nm). Longer Debye lengths led to poorer SPR responses, revealing a reduced affinity of the folic acid-modified Au NPs for hDHFR. While high colloidal stability of Au NPs is desired in most applications, these conditions may hinder molecular interactions due to Debye lengths exceeding the size of the ligand and thus preventing close interactions with the surface-bound molecular receptor.

Graphical abstract: Influence of the Debye length on the interaction of a small molecule-modified Au nanoparticle with a surface-bound bioreceptor

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The article was received on 24 Feb 2014, accepted on 30 Mar 2014 and first published on 31 Mar 2014


Article type: Communication
DOI: 10.1039/C4CC01423E
Citation: Chem. Commun., 2014,50, 4947-4950
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    Influence of the Debye length on the interaction of a small molecule-modified Au nanoparticle with a surface-bound bioreceptor

    N. Bukar, S. S. Zhao, D. M. Charbonneau, J. N. Pelletier and J. Masson, Chem. Commun., 2014, 50, 4947
    DOI: 10.1039/C4CC01423E

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