Issue 20, 2014

Fatty acid profiles reveal toxic responses in adipose tissue of C57BL/6J mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin

Abstract

Dioxin exposure tends to accumulate in adipose tissue and alters metabolism in mammals. In this study, gas chromatography coupled with mass spectrometry (GC-MS) in conjunction with multivariate statistical analysis was applied to profile small molecular metabolites in adipose tissue of aryl hydrocarbon receptor (AhR)-high affinity wild-type C57BL/6J mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). A partial least squares discriminant analysis model was also constructed to map the discrimination between TCDD dosages and the control group. As a result, a total of 16 differential metabolites were identified in the high-dose TCDD group compared to the control group, and 12 free fatty acids (FFAs) were highlighted among them. Both saturated and unsaturated FFA levels were significantly elevated in adipose tissues of TCDD-exposed mice. This promising tool for global characterization highlights FFAs which could be served as indicators for understanding the toxic responses to TCDD exposure in a dose-dependent manner. The data indicated that the use of GC-MS coupled with multivariate statistical analysis could provide new insight for fatty acid biosynthesis on AhR activation with TCDD exposure in wild-type mice.

Graphical abstract: Fatty acid profiles reveal toxic responses in adipose tissue of C57BL/6J mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin

Article information

Article type
Paper
Submitted
22 Jun 2014
Accepted
19 Aug 2014
First published
19 Aug 2014

Anal. Methods, 2014,6, 8207-8211

Author version available

Fatty acid profiles reveal toxic responses in adipose tissue of C57BL/6J mice exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin

C. Hu, S. Lin and Z. Cai, Anal. Methods, 2014, 6, 8207 DOI: 10.1039/C4AY01479K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements