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Issue 20, 2014
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A broad-selective enzyme immunoassay for non-invasive stress assessment in African penguins (Spheniscus demersus) held in captivity

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Abstract

We applied a direct competitive immunoassay for measuring corticosterone and glucocorticoid metabolites in faeces (FGMs) as a non-invasive tool for monitoring the stress response of African penguins (Spheniscus demersus) held in captivity in a zoological facility. The developed assay, validated in-house, proved to be rapid (the test could be completed in 90 minutes), sensitive (LOD for corticosterone 0.2 μg l−1, dynamic range 0.75–75 μg l−1) and broad-selective, as it cross-reacted with the major corticosteroids, thus allowing the detection of excreted FGMs resulting from a biological stressor. Matrix interference, due to components of faecal samples, was overcome by diluting sample extracts (1 + 4 or 1 + 9, depending on the sample). The assay enabled us to investigate the response to stress in five animals – three adult males and two adult females – over a period of 30 hours, and to identify the peak of FGM production as being 7–10 hours after the stressful event.

Graphical abstract: A broad-selective enzyme immunoassay for non-invasive stress assessment in African penguins (Spheniscus demersus) held in captivity

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Publication details

The article was received on 12 Jun 2014, accepted on 30 Jul 2014 and first published on 30 Jul 2014


Article type: Paper
DOI: 10.1039/C4AY01407C
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Citation: Anal. Methods, 2014,6, 8222-8231
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    A broad-selective enzyme immunoassay for non-invasive stress assessment in African penguins (Spheniscus demersus) held in captivity

    L. Anfossi, L. Ozella, F. Di Nardo, C. Giovannoli, C. Passini, L. Favaro, D. Pessani, E. Mostl and C. Baggiani, Anal. Methods, 2014, 6, 8222
    DOI: 10.1039/C4AY01407C

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