Application of an ELISA-type amperometric assay to the detection of Vibrio species with screen-printed electrodes
Abstract
We describe an electrochemical method for the rapid detection of Vibrio species. An avidin–biotin binding strategy was tested with 12 different Vibrio strains and applied onto gold screen-printed electrodes. V. parahaemolyticus cells were then amperometrically detected at a minimum abundance of 4 × 102V. parahaemolyticus cells per mL within 60 min.