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Issue 35, 2014
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Cross-catalytic peptide nucleic acid (PNA) replication based on templated ligation

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Abstract

We report the first PNA self-replicating system based on template directed cross-catalytic ligation, a process analogous to biological replication. Using two template PNAs and four pentameric precursor PNAs, all four possible carbodiimide assisted amide ligation products were detected and identified by HPLC and MALDI-TOF analysis. We conclude that the two template complementary reaction products are generated via cross-catalysis, while the other two self-complementary (and in principle auto-catalytic) products are formed via intra-complex coupling between the two sets of complementary PNA precursors. Cross-catalytic product formation followed product inhibited kinetics, but approximately two replication rounds were observed. Analogous but less efficient replication was found for a similar tetrameric system. These results demonstrate that simpler nucleobase replication systems than natural oligonucleotides are feasible, thereby strengthening the foundation for the discussion of a possible role for PNA (like) genetic material in the prebiotic evolution of life and lay the ground for further studies into evolution of such potentially prebiotic systems.

Graphical abstract: Cross-catalytic peptide nucleic acid (PNA) replication based on templated ligation

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Supplementary files

Article information


Submitted
04 Jun 2014
Accepted
17 Jul 2014
First published
17 Jul 2014

This article is Open Access

Org. Biomol. Chem., 2014,12, 6901-6907
Article type
Paper
Author version available

Cross-catalytic peptide nucleic acid (PNA) replication based on templated ligation

A. Singhal and P. E. Nielsen, Org. Biomol. Chem., 2014, 12, 6901
DOI: 10.1039/C4OB01158A

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